Establishment of background-free RNA proximity labelling for low abundant RNA-protein complexes
Advisor: Ralf-Peter Jansen
PhD Program: International Max Planck Research School (IMPRS) 'From Molecules to Organisms'
Location: University of Tübingen
Proximity labelling is a new biochemical approach that allows the identification of bait-associated proteins or RNAs by biotinylation in vivo. The technique has been widely applied to protein complexes as well as RNA-protein assemblies (RNPs, RNA-protein particles). However, most RNPs are very dynamic and can undergo rapid changes in their protein partners. Hence, the application of proximity labelling techniques for the characterization of RNPs requires certain modifications, e.g. increase in labelling efficiency or reduction of labelling times. The prospective student will develop a method for background-free proximity labelling of RNA-bound proteins, using beta-actin mRNA and NORAD lncRNA as RNA models in cultured stem cells and fibroblasts.
More information about the research of Ralf-Peter Jansen and a selection of recent publications can be found on his faculty page.
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Application deadline: 8 February 2023