Systems Genetics of Evolution
Friedrich Miescher Laboratory
Faculty in: TIPP, IMPRS
- PhD in the Department of Developmental Biology, Stanford University, 2003-09
- Postdoctoral training, Max Planck Institute for Evolutionary Biology, Plön, 2009-12
- Max Planck Research Group Leader at the FML since 2012
The genome is in constant evolution. Despite this on-going change, the genome also has to maintain essential functions. Discovering the evolutionary process underlying genome evolution is thus a central goal not only in evolutionary genetics, but also in medicine. Using the house mouse as our model organism, we aim to connect molecular mutations to their phenotypic consequences and track their fate through evolution.
We have pioneered an innovative system to induce mitotic recombination in mouse embryonic stem cells, which allows us to perform genetic mapping for the first time between sister mouse species directly in pluripotent cells. By creating interspecific hybrids between mice with increasing divergence, we can study how traits evolve across species boundaries. Using the in vitro cross system, we can now generate diversity in a single petri dish and directly track how reshuffled genetic combinations may lead to gene expression or cell fate differences between cells. Our in vitro cross approach creates “Impossible Hybrids” – advanced recombinant cell lines that circumvent conventional species barriers.
Our group employs the latest functional genomics and tissue culture techniques, including single-cell sorting and droplet microfluidics techniques to draw out the unique features of every single cell. Our overarching aim is to construct gene networks across evolutionary divergences and connect the dots between individual genetic changes and their evolutionary impacts.
The ultimate goal of our research is to understand how evolution has shaped gene regulatory networks to be tightly, yet flexibly interwoven whole that simultaneously exhibit robustness and a capacity for rapid adaptation.
Available PhD Projects
- Currently not recruiting PhD students.
- Meier, J.I.*, Salazar, P.A.*, Kučka, M.*, Davies, R.W., Dréau, A., Aldás, I., Box-Power, O., Nadeau, N., Bridle, J.R., McMillan, W.O., Rolian, C.P., Jiggins, C.D.†, Chan, Y.F.† Haplotype tagging reveals parallel formation of hybrid races in two butterfly species. (* co-first authors; † co-last authors). In revision at PNAS, also bioRxiv, doi: 10.1101/2020.05.25.113688.
- Lazzarano S, Kučka M, Castro JPL, Naumann R, Medina P, et al. (2018) Genetic mapping of species differences via in vitro crosses in mouse embryonic stem cells. PNAS. doi: 10.1073/pnas.1717474115.
- Castro JPL, Yancoskie MN, Marchini M, Belohlavy S, Kučka M, et al. (2018) An integrative genomic analysis of the Longshanks selection experiment for longer limbs in mice. bioRxiv, doi: 10.1101/378711.