miRNAs as Mobile Signals in Development
University of Tübingen
Faculty in: IMPRS
- PhD studies at Rutgers University, 1990-1996
- Postdoctoral training at Yale University, 1996-1998
- Assistant, Associate, and Full Professor at Cold Spring Harbor Laboratory, 1998-2015
- Alexander von Humboldt Professor at the University of Tübingen since 2015
The formation of stable, precisely defined boundaries between two distinct cell fates is a fundamental feature of plant and animal development. Such cell fate boundaries coordinate the differentiation and growth of the tissue or organ. In this regard, development of flat leaf architecture poses an unusual and mechanistically challenging problem; namely, how to create a stable dorsoventral (top-bottom) boundary within the plane of a long and wide, but shallow, structure? We have shown that the positional information needed is provided by mobile small RNAs that reminiscent to classical morphogens generate sharply defined domains of target gene expression through an intrinsic, threshold-based readout of their mobility gradients (Skopelitis et al., 2017).
Major questions regarding the properties and function of mobile small RNAs in development, however, remain: how do small RNAs move, how is their mobility regulated, what are the distinguishing patterning properties of mobile small RNAs, and how might opposing signal gradients interact to achieve the remarkable precision and robustness of developmental programs? We combine classical genetics with single cell genomic approaches, quantitative time-lapse imaging and theoretical modelling to address these questions in the model plant Arabidopsis. Please see here for more detail.
Available PhD Projects
Currently not recruiting PhD students
- Skopelitis, D.S., Hill, K., Klesen, S., Marco, C.F., von Born, P., et al. (2018) Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals. Nat Commun. 9, 3107, doi:10.1038/s41467-018-05571-0.
- Skopelitis, D.S., Benkovics, A.H., Husbands, A.Y., and Timmermans, M.C.P. (2017) Boundary formation through a direct threshold-based readout of mobile small RNA gradients. Dev Cell 43, 265-273, doi: 10.1016/j.devcel.2017.10.003.
- Denyer, T., Ma, X., Klesen, S., Scacchi, E., Nieselt, K., and Timmermans M.C.P. (2019) Spatiotemporal developmental trajectories in the Arabidopsis root revealed using high-throughput single cell RNA sequencing. Dev Cell 48, 840-852, doi: 10.1016/j.devcel.2019.02.022 .